Differentiation of mouse embryonal carcinoma cells PCC4 by heat shock and the kinetics of induction of heat shock proteins.
Indian J Biochem Biophys
;
1994 Aug; 31(4): 295-301
Artigo
em Inglês
| IMSEAR
| ID: sea-27671
ABSTRACT
Heat shock to embryonal carcinoma cells PCC4 at 45 degrees C for 30 min resulted in the differentiation of cells although heat shock response was induced on exposure to 42 degrees C for 60 min. Differentiated cells were large and well spread with reduced nuclear/cytoplasmic ratios as compared to undifferentiated cells. Change in cell morphology was associated with the disappearance and appearance of stage specific embryonic antigens 1 and 3 respectively. We also found a change in intracellular pH in PCC4 cells within 30 min of heat shock as measured by the change in fluorescence intensity of a probe incorporated into cells during heat shock.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Células Tumorais Cultivadas
/
Cinética
/
Diferenciação Celular
/
Carcinoma Embrionário
/
Temperatura Alta
/
Proteínas de Choque Térmico
/
Animais
/
Camundongos
Idioma:
Inglês
Revista:
Indian J Biochem Biophys
Ano de publicação:
1994
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS