Modulation of monkey small intestinal brush border membrane D-glucose transport by nonesterified fatty acids.
Indian J Biochem Biophys
;
1993 Jun; 30(3): 172-6
Artigo
em Inglês
| IMSEAR
| ID: sea-27673
ABSTRACT
Brush border membranes isolated from monkey intestinal mucosa was found to contain considerable amount of nonesterified fatty acids. Incubation of brush border membranes with fatty acid free albumin selectively removed the free fatty acids more than 80% without altering the level of phospholipids or cholesterol. The sodium dependent D-glucose transport was stimulated by the albumin treatment. Kinetic study showed that albumin treatment did not alter the apparent affinity (Km) of the transporter for glucose whereas the maximal velocity (Vmax) was increased significantly. The sodium dependent D-glucose transport was inhibited by the exogenously added unsaturated fatty acids. Saturated fatty acids and methyl esters of unsaturated fatty acids showed no inhibition. Based on these results, it may be concluded that free fatty acids inhibit the sodium dependent intestinal D-glucose transport either by directly interacting with the transport protein or by abolishing the sodium gradient.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Transporte Biológico
/
Cinética
/
Ácidos Graxos não Esterificados
/
Ácidos Graxos Insaturados
/
Glucose
/
Mucosa Intestinal
/
Intestino Delgado
/
Animais
/
Macaca radiata
/
Microvilosidades
Idioma:
Inglês
Revista:
Indian J Biochem Biophys
Ano de publicação:
1993
Tipo de documento:
Artigo
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