Identification of a non-microsomal cinnamic acid 4-hydroxylase from potato tuber (S. tuberosum) and its partial purification.
Indian J Biochem Biophys
;
1992 Oct; 29(5): 418-24
Artigo
em Inglês
| IMSEAR
| ID: sea-27787
ABSTRACT
The cytoplasmic localisation of cinnamic acid 4-hydroxylase (CA4H) has been shown by isolation and subcellular fractionation of the enzyme in Hepes buffer. The enzyme was purified by ammonium sulphate fractionation followed by AcA-34 molecular sieve chromatography. The enzyme existed as a high molecular mass which dissociated to a lower form on dilution on the column. The pH optimum, sulphydryl requirement, molecular and preliminary kinetic characteristics were investigated.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Frações Subcelulares
/
Solanum tuberosum
/
Cinética
/
Cátions Bivalentes
/
Fracionamento Celular
/
Sistema Enzimático do Citocromo P-450
/
Transcinamato 4-Mono-Oxigenase
/
Concentração de Íons de Hidrogênio
/
Oxigenases de Função Mista
/
Microssomos
Tipo de estudo:
Estudo prognóstico
Idioma:
Inglês
Revista:
Indian J Biochem Biophys
Ano de publicação:
1992
Tipo de documento:
Artigo
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