Ultrastructural localization of gangliosides, calcium and a high-affinity Ca(2+)-ATPase in nerve terminals: a contribution to the possible functional role of gangliosides.
Indian J Biochem Biophys
;
1990 Dec; 27(6): 420-4
Artigo
em Inglês
| IMSEAR
| ID: sea-27809
ABSTRACT
By means of newly developed electron microscopical techniques (electron spectroscopic imaging, ESI; electron energy loss spectroscopy, EELS; immunogold labelling) a specific accumulation of endogenous calcium within the synaptic cleft and a distinct localization of a high-affinity Ca(2+)-ATPase at the inner sides of the pre- and postsynaptic membrane of nerve cells from fish brain have been demonstrated. Additionally, a differentiation-dependent expression of polysialoganglioside epitopes on the outer surface of nerve terminals in clustered arrangements was demonstrated using their ultracytochemical detection by means of the monoclonal antibody Q211. These results which are in agreement with parallel biochemical investigations on modulatory effects of exogenous gangliosides on a high-affinity Ca(2+)-ATPase in the CNS of vertebrates support our hypothesis that Ca(2+)-ganglioside complexes act as modulators for the processes of synaptic transmission and long-term neuronal adaptations.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Sinapses
/
Cálcio
/
Microscopia Imunoeletrônica
/
ATPases Transportadoras de Cálcio
/
Transmissão Sináptica
/
Neurotransmissores
/
Peixes
/
Gangliosídeos
/
Animais
/
Anticorpos Monoclonais
Idioma:
Inglês
Revista:
Indian J Biochem Biophys
Ano de publicação:
1990
Tipo de documento:
Artigo
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