Ficus cunia agglutinin for recognition of bacteria.
Indian J Biochem Biophys
;
2006 Apr; 43(2): 94-7
Artigo
em Inglês
| IMSEAR
| ID: sea-28055
ABSTRACT
Interaction of bacteria with lectin using anti-lectin antibody by ELISA is an established method. In the present study, we have devised a simple ELISA using a biotinylated lectin and antibiotin-HRP. Ficus cunia agglutinin (FCA), which has shown the specificity towards alpha/beta anomers of GlcNAc and other-NAc containing sugars like LacNAc and GlcNAcbeta(1-4/6)GlcNAc, was used as a model lectin for the study of interaction with immobilized microorganisms on ELISA plate. The bacterial cells of E. coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Bacillus subtilis and Staphylococcus aureus showed binding with FCA and the degree of binding was dependent on the bacterial surface antigen. This method is considered a simple technique to study the lectin-bacteria interaction.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Ensaio de Imunoadsorção Enzimática
/
Ficus
/
Lectinas de Plantas
/
Bactérias Gram-Negativas
/
Bactérias Gram-Positivas
Idioma:
Inglês
Revista:
Indian J Biochem Biophys
Ano de publicação:
2006
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS