A sensitive assay for Staphylococcus aureus nucleases.
Indian J Biochem Biophys
;
1989 Oct; 26(5): 296-300
Artigo
em Inglês
| IMSEAR
| ID: sea-28082
ABSTRACT
A sensitive assay for staphylococcal nuclease involving incubation of the enzyme sample with heat-denatured [3H]thymidine labelled DNA from E. coli, precipitation with trichloroacetic acid and measurement of the radioactivity of acid-soluble nucleotides released has been developed. The assay is sensitive enough to be used for comparing the levels of nucleases elaborated by different strains of S. aureus as well as for determining the extent of contamination of S. aureus in food and water samples even at levels at which the conventional spectrophotometric and toluidine blue-DNA methods are totally inadequate.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Staphylococcus aureus
/
Cinética
/
Desoxirribonucleases
Idioma:
Inglês
Revista:
Indian J Biochem Biophys
Ano de publicação:
1989
Tipo de documento:
Artigo
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