Reversible inhibition by pyridoxal 5'-phosphate and irreversible inactivation by urea and guanidine hydrochloride of thymidylate synthase from Lactobacillus leichmannii.
Indian J Biochem Biophys
;
1998 Aug; 35(4): 229-35
Artigo
em Inglês
| IMSEAR
| ID: sea-28159
ABSTRACT
Pyridoxal 5'-phosphate reversibly inhibited thymidylate synthase from Lactobacillus leichmannii. The inhibition was competitive with dUMP (Ki = 1 microM) and non-competitive with 5,10-CH2-THF (Ki = 0.08 microM). Treatment of native or pCMB-treated enzyme with urea (5 M) or guanidine hydrochloride (4 M) resulted in inactivation and dissociation of the homodimer (74 kDa) into monomer (37 kDa).
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Conformação Proteica
/
Fosfato de Piridoxal
/
Timidilato Sintase
/
Ureia
/
Dimerização
/
Guanidina
/
Inibidores Enzimáticos
/
Lactobacillus
Idioma:
Inglês
Revista:
Indian J Biochem Biophys
Ano de publicação:
1998
Tipo de documento:
Artigo
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