Heterogeneity of transport systems for L-glutamine in mouse mammary gland.
Indian J Biochem Biophys
;
2001 Aug; 38(4): 241-8
Artigo
em Inglês
| IMSEAR
| ID: sea-28281
ABSTRACT
The characteristics of the transport systems of L-glutamine in lactating mouse mammary gland have been studied. L-glutamine uptake was mediated by three Na+-dependent and one Na+-independent systems. The 2-(methylamino)isobutyric acid-sensitive component of Na+-dependent uptake exhibited the usual characteristics of system A. The other two Na+-dependent systems, which we have named BCI(-)-dependent and BCl(-)-independent, are the new systems identified. These are broad specificity systems and were discriminated on the basis of inhibition analysis, Cl- dependency and the effect of preloading mammary tissue with amino acids. While L-aspargine inhibited the uptake of L-glutamine via both these broad specificity systems, L-homoserine inhibited the uptake of L-glutamine via only BCl(-)-dependent system. The uptake of L-glutamine via the BCl(-)-independent system was upregulated by preloading mammary tissue with L-serine, while BCl(-)-dependent system was unaffected. The Na+-independent uptake of L-glutamine was inhibited by 2-aminobicyclo-(2,2,1)heptane carboxylic acid and other neutral amino acids, and identified as the system L.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Técnicas de Cultura de Órgãos
/
Sódio
/
Transporte Biológico
/
Feminino
/
Lactação
/
Cinética
/
Aminoácidos Cíclicos
/
Glutamina
/
Concentração de Íons de Hidrogênio
/
Animais
Tipo de estudo:
Estudo prognóstico
Idioma:
Inglês
Revista:
Indian J Biochem Biophys
Ano de publicação:
2001
Tipo de documento:
Artigo
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