Kinetic properties and storage stability of catalase immobilized on to florisil.
Indian J Biochem Biophys
;
2007 Feb; 44(1): 38-43
Artigo
em Inglês
| IMSEAR
| ID: sea-28315
ABSTRACT
The covalent immobilization of bovine liver catalase (CAT) on to florisil via glutaraldehyde was investigated. Optimum immobilization pH and temperature were determined as pH 6.0, 10 degrees C respectively, while the amount of initial CAT per g of carrier and immobilization time was determined as 5 mg g(-1) and 120 min, respectively. The Vmax values for free and immobilized CAT were found to be 1.7 x 10(5) and 2.0 x 10(4) micromol H2O2 min(-1) mg protein(-1), respectively, whereas KM values were 33.3 mM and 1722.0 mM respectively. Operational stability was determined by using a stirred batch-type column reactor. Immobilized CAT retained about 40% of its initial activity after 50 uses. It showed higher storage stability than free CAT at 4 degrees C and 25 degrees C. Its storage stability increased with increasing relative humidity (RH) from 0 to 20% of the medium. The highest storage stability was obtained in 20% RH, however, further increase in RH from 40 to 100% significantly decreased the storage stability.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Estabilidade Enzimática
/
Soluções Tampão
/
Bovinos
/
Cinética
/
Catalase
/
Silicatos de Magnésio
/
Armazenamento de Medicamentos
/
Enzimas Imobilizadas
/
Concentração de Íons de Hidrogênio
/
Fígado
Idioma:
Inglês
Revista:
Indian J Biochem Biophys
Ano de publicação:
2007
Tipo de documento:
Artigo
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