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Effects of monensin on glycosphingolipid metabolism in cultured human proximal tubular cells.
Indian J Biochem Biophys ; 1993 Dec; 30(6): 346-52
Artigo em Inglês | IMSEAR | ID: sea-28765
ABSTRACT
Effects of monensin, a monovalent cationic ionophore which disrupts Golgi apparatus and its related functions, on glycosphingolipid (GSL) metabolism were investigated in cultured human proximal tubular (PT) cells. Monensin (10(-6) M) stimulated [3H]Gal incorporation into GlcCer, GalCer and LacCer by 8.5-fold and 15-fold, respectively, in PT cells as compared to control. In contrast, [3H]Gal incorporation into GbOse3Cer and GM3 remained unchanged and that into GbOse4Cer was decreased 2-fold as compared to control. GSL measured by HPLC revealed that in cells incubated with monensin, GlcCer, GalCer and LacCer levels were increased 1.6-fold and 7-fold, respectively, whereas GbOse3Cer and GbOse4Cer levels were decreased several folds. Cells incubated with monensin contained 2.5- to 3-fold higher activity of alpha-galactosidase, beta-galactosidase and beta-glucosidase than control, whereas the activity of UDP-gal glucosylceramide galactosyltransferase (beta-GalT-2) was 8-fold lower than control cells. Cells incubated with monensin took up and degraded one-half as much 125I-LDL as that of control cells. In control cells, exogenously derived [3H]LacCer on LDL was rapidly taken up and catabolized to monoglycosylceramide, or it was used for the endogenous synthesis of globotriosylceramide (trihexosylceramide), globotetraosylceramide (tetrahexosylceramide) and a ganglioside, GM3. In contrast, cells incubated with monensin accumulated most of the [3H]LacCer-LDL. Exogenously derived [3H]LacCer on LDL was catabolized to GlcCer, but was not utilized, for the synthesis of globotriosylceramide, globotetraosylceramide and GM3 in cells incubated with monensin.(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Texto completo: DisponíveL Índice: IMSEAR (Sudeste Asiático) Assunto principal: Humanos / Glicoesfingolipídeos / Monensin / Linhagem Celular / Células Cultivadas / Beta-Galactosidase / Beta-Glucosidase / Fibroblastos / Galactose / Galactosiltransferases Idioma: Inglês Revista: Indian J Biochem Biophys Ano de publicação: 1993 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: IMSEAR (Sudeste Asiático) Assunto principal: Humanos / Glicoesfingolipídeos / Monensin / Linhagem Celular / Células Cultivadas / Beta-Galactosidase / Beta-Glucosidase / Fibroblastos / Galactose / Galactosiltransferases Idioma: Inglês Revista: Indian J Biochem Biophys Ano de publicação: 1993 Tipo de documento: Artigo