In vitro and in vivo inhibition of telomerase activity from Chinese hamster V79 cells.
Indian J Biochem Biophys
;
2001 Feb-Apr; 38(1-2): 42-7
Artigo
em Inglês
| IMSEAR
| ID: sea-28864
ABSTRACT
While studying the inhibition of telomerase activity in Chinese hamster V79 cells using polymerase chain reaction (PCR) based telomeric repeat amplification protocol (TRAP) assay, we had earlier observed that 7-deaza deoxy guanosine triphosphate (7-deaza dGTP) and oligonucleotide (TTAGGG)4 inhibited telomerase activity in vitro. In the present study, we report inhibition of telomerase activity by modified base 7-deaza deoxy adenosine triphosphate (7-deaza dATP) and phosphorothioate TTAGGG (PS-TTAGGG). Both the compounds inhibited telomerase activity in a concentration dependent manner; 8.5 microM of 7-deaza dATP and 0.1 microM of PS-TTAGGG being the concentration for 50% of the maximum inhibition. This observation supports our earlier hypothesis that incorporation of a modified nucleotide into telomere possibly interferes with the recognition of the telomerase and TTAGGG interferes with the RNA component of telomerase. We have further shown that treatment of cells with nicotinamide (NA) and benzamide (BA), well known inhibitors of poly (ADP-ribose) polymerase, reduced telomerase activity. We speculate that modification of the telomeric binding proteins or other components by poly (ADP-ribosyl)ation may be involved in such inhibition.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Ligação Proteica
/
Benzamidas
/
Linhagem Celular
/
Reação em Cadeia da Polimerase
/
Cricetulus
/
Cricetinae
/
Telômero
/
Niacinamida
/
Telomerase
/
Densitometria
Tipo de estudo:
Guia de Prática Clínica
Idioma:
Inglês
Revista:
Indian J Biochem Biophys
Ano de publicação:
2001
Tipo de documento:
Artigo
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