Isolation, purification and preliminary studies on fish eye lens glycoprotein.
Indian J Biochem Biophys
;
1991 Feb; 28(1): 63-4
Artigo
em Inglês
| IMSEAR
| ID: sea-28960
ABSTRACT
Glycoprotein from the eye lens of fish, Mystus cavasius, was isolated by extraction with 1% Triton X-100 in saline. The crude extract which was found to be electrophoretically heterogeneous was fractionated on a DEAE-cellulose column. One of the fractions obtained in major amount was further resolved by column chromatography using Sephadex G-150 into two homogeneous fractions (GP-1 and GP-2]. GP-1 contained carbohydrates (11.2%) and protein (77.5%). The constituent sugars were D-glucose, D-mannose, D-galactosamine and N-acetyl neuraminic acid. The principal amino acids were aspartic acid, serine, glutamic acid, glycine and alanine. The proportions of these residues were determined.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Polietilenoglicóis
/
Carboidratos
/
Glicoproteínas
/
Cromatografia DEAE-Celulose
/
Octoxinol
/
Proteínas do Olho
/
Peixes
/
Aminoácidos
/
Cristalino
/
Animais
Idioma:
Inglês
Revista:
Indian J Biochem Biophys
Ano de publicação:
1991
Tipo de documento:
Artigo
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