Isatin (2,3-dioxoindole): a competitive inhibitor of Na(+)-dependent lysine uptake in rat instestine.
Indian J Biochem Biophys
;
1996 Dec; 33(6): 519-22
Artigo
em Inglês
| IMSEAR
| ID: sea-28963
ABSTRACT
Isatin (2,3-dioxoindole) competitively inhibited (27-40%) Na(+)-dependent L-lysine uptake in rat intestine. The value of Kt was increased from 3.04 mM in control to 5.88 mM in presence of 10 mM isatin. Effect of isatin on the Na(+)-independent amino acid uptake was insignificant (12-18%). The inhibitory constant (Ki) was 2.8 mM under these conditions. The observed inhibition was unaffected by -SH group reacting agents. Isatin (1-10 mM) inhibited Na+, K(+)-ATPase activity in intestine in vitro, the maximum inhibition (66%) being at 10 mM isatin concentration. But the drug had no effect on enzyme activity under in vivo conditions.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Ratos
/
Sódio
/
Reagentes de Sulfidrila
/
Transporte Biológico
/
Cinética
/
ATPase Trocadora de Sódio-Potássio
/
Mucosa Intestinal
/
Isatina
/
Animais
/
Lisina
Idioma:
Inglês
Revista:
Indian J Biochem Biophys
Ano de publicação:
1996
Tipo de documento:
Artigo
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