Analysis of the activity of promoters from two photosynthesis-related genes psaF and petH of spinach in a monocot plant, rice.
Indian J Biochem Biophys
;
2000 Dec; 37(6): 447-52
Artigo
em Inglês
| IMSEAR
| ID: sea-28972
ABSTRACT
The subunit III of photosystem I and ferredoxin-NADP(+)-oxidoreductase are encoded by nuclear genes, namely psaF and petH. The activity of their promoters from spinach has been evaluated in transgenic tobacco earlier. Evaluation of the activity of these Dicotyledoneae-specific promoters has been carried out in a monocot system (i.e. rice) by transient gene expression system, based on electroporation-mediated gene delivery into protoplasts from leaves and roots. It has been found that various promoter deletions show higher activity in leaf protoplasts and elements for quantitative response are widely distributed. Transgenic rice has also been produced with a petH promoter and gus reporter gene construct. Although petH promoter is a weak promoter in comparison to the 35S promoter, it expresses well in green tissues and could be useful for plant genetic engineering.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Fotossíntese
/
Proteínas de Plantas
/
Oryza
/
Proteínas de Bactérias
/
Regiões Promotoras Genéticas
/
Plantas Geneticamente Modificadas
/
Genes Reporter
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Spinacia oleracea
/
Complexo de Proteína do Fotossistema I
/
Ferredoxina-NADP Redutase
Idioma:
Inglês
Revista:
Indian J Biochem Biophys
Ano de publicação:
2000
Tipo de documento:
Artigo
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