Hormonal modulation of secretion of immunoreactive riboflavin carrier protein by adult rat Leydig cells in vitro.
Indian J Biochem Biophys
;
1996 Aug; 33(4): 274-80
Artigo
em Inglês
| IMSEAR
| ID: sea-29099
ABSTRACT
Adult rat Leydig cells in culture synthesize and secrete riboflavin carrier protein (RCP) as demonstrated by [35S]-methionine incorporation into newly synthesized proteins followed by immunoprecipitation as well as specific radioimmunoassay. LH stimulates the secretion of RCP 4-fold which could be inhibited upto 75% by an aromatase inhibitor. 8-bromo-cyclic AMP and cholera toxin could mimic the LH stimulated secretion of the carrier protein. The extent of stimulation of RCP secretion brought about by exogenous estradiol-17 beta is comparable to that of LH. The antiestrogen tamoxifen, when added along with either LH or estrogen, inhibited the stimulated levels significantly. These results show that the estrogen-inducible riboflavin carrier is secreted by Leydig cells under positive regulation of LH.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Proteínas de Membrana Transportadoras
/
Ratos
/
Riboflavina
/
Tamoxifeno
/
8-Bromo Monofosfato de Adenosina Cíclica
/
Masculino
/
Hormônio Luteinizante
/
Proteínas de Transporte
/
Ratos Wistar
/
Estradiol
Idioma:
Inglês
Revista:
Indian J Biochem Biophys
Ano de publicação:
1996
Tipo de documento:
Artigo
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