Preparation of a neutralizing monoclonal antibody specific to serotype 8 rotavirus strains with a super-short RNA pattern.
Southeast Asian J Trop Med Public Health
;
1991 Mar; 22(1): 41-5
Artigo
em Inglês
| IMSEAR
| ID: sea-30528
ABSTRACT
Extensive cross-neutralization tests have identified seven serotypes of rotavirus. Recently, it has been reported that several human strains (69M, B37 and B38) with a super-short RNA pattern carry new serotype specificity (serotype 8). By using the strain 69M as an immunizing antigen, we prepared a neutralizing monoclonal antibody (designated 69M-2D). In neutralization tests using 18 human and two animal rotavirus strains with different serotype specificities, the 69M-2D antibody was found to neutralize only serotype 8 strains. Also, in an enzyme-linked immunosorbent assay (ELISA), the antibody reacted exclusively with the serotype 8 strains. Thus, the reactivity pattern of the 69M-2D antibody was serotype 8-specific. This antibody should be useful as another reagent for serotyping rotaviruses by ELISA.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
RNA de Cadeia Dupla
/
RNA Viral
/
Ensaio de Imunoadsorção Enzimática
/
Testes de Neutralização
/
Genoma Viral
/
Rotavirus
/
Animais
/
Camundongos
/
Camundongos Endogâmicos BALB C
/
Anticorpos Monoclonais
Idioma:
Inglês
Revista:
Southeast Asian J Trop Med Public Health
Ano de publicação:
1991
Tipo de documento:
Artigo
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