Multi-dot enzyme-linked immunosorbent assay for serodiagnosis of trematodiasis.
Southeast Asian J Trop Med Public Health
;
1990 Sep; 21(3): 471-4
Artigo
em Inglês
| IMSEAR
| ID: sea-30971
ABSTRACT
A nitrocellulose membrane strip dotted with crude antigens of trematodes, such as Paragonimus miyazakii, P. westermani, Fasciola sp., Clonorchis sinensis and Schistosoma japonicum was applied for serodiagnosis of trematodiasis. With peroxidase conjugated anti-human IgG and 4-chloro-1-naphthol as a substrate, antibodies in trematodiasis patient sera were clearly detected on the strip as bluish purple spots. Densities of the spots correlated well with values obtained by standard micro-ELISA. Cross-reactions among the trematode antigens, which is occurred more frequently than by ELISA, were observed with this method. However, the darkest spot correlated with the infecting parasite in all cases. With the high reliability and advantage that crude antigens can be used, this simple method, multi-dot ELISA, is considered to be useful for serodiagnosis of trematodiasis.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Trematódeos
/
Infecções por Trematódeos
/
Ensaio de Imunoadsorção Enzimática
/
Testes Sorológicos
/
Anticorpos Anti-Helmínticos
/
Reações Cruzadas
/
Animais
Tipo de estudo:
Estudo diagnóstico
Idioma:
Inglês
Revista:
Southeast Asian J Trop Med Public Health
Ano de publicação:
1990
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS