Killing of blood stage Plasmodium vinckei petteri by spleen macrophages through L-arginine dependent mechanism.
Southeast Asian J Trop Med Public Health
;
1997 Sep; 28(3): 489-95
Artigo
em Inglês
| IMSEAR
| ID: sea-30993
ABSTRACT
A series of experiments was carried out to investigate the involvement of the L-arginine-dependent effector mechanism (LADEM) in the killing of the blood stages of the rodent malaria parasite, Plasmodium vinckei petteri, by activated spleen macrophages in vitro. P.v.petteri-infected red blood cells were co-incubated with spleen macrophages from normal mice which had previously received 10(8) Mycobacterium bovis (BCG) 5 days earlier, in the presence of 0.1 microgram/ml LPS with and without 0.1 mM L-NMMA, an L-arginine analogue which inhibits LADEM, for 16 hours. The viability of the parasites was assessed according to their infectivity following inoculation into experimental mice. Incubation of parasites with spleen macrophages in the presence of LPS without L-NMMA reduced the parasite viability to about 3%. When L-NMMA was included in the culture, inhibition of parasite killing was observed, resulting in an increase of parasite viability to about 21%. These data provide evidence to suggest that spleen macrophages play an important role as effector cells in the immune mechanisms against P.v.petteri infection, and that the parasite killing of these cells, at least in part, was mediated by LADEM.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Arginina
/
Plasmodium
/
Roedores
/
Baço
/
Fatores de Tempo
/
Feminino
/
Vacina BCG
/
Células Cultivadas
/
Lipopolissacarídeos
/
ômega-N-Metilarginina
Tipo de estudo:
Estudo prognóstico
Idioma:
Inglês
Revista:
Southeast Asian J Trop Med Public Health
Ano de publicação:
1997
Tipo de documento:
Artigo
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