Baculovirus expression of the major surface antigen of Toxoplasma gondii and the immune response of mice injected with the recombinant P30.
Southeast Asian J Trop Med Public Health
;
1999 Mar; 30(1): 42-6
Artigo
em Inglês
| IMSEAR
| ID: sea-31107
ABSTRACT
The major surface antigen (P30) of the Toxoplasma gondii was expressed by an insect cell culture system infected with recombinant baculovirus. About 750 microg of purified (95% purity) P30 was obtained from a culture of 10(8) insect Sf21 cells. The recombinant P30 was used to immunize mice to induce immune response. Mice injected with the recombinant protein produced specific humoral and cellular immune responses. Immunization with P30 also prolonged the period of survival of mice infected by Toxoplasma. The average survival time of control group is 13.25+/-1.16 days, but are 16.13+/-2.1 days, 19.50+/-3.21 days, 20.38+/-3.38 days in different immunized groups, respectively.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Toxoplasma
/
Anticorpos Antiprotozoários
/
Transfecção
/
Regulação Viral da Expressão Gênica
/
Proteínas de Protozoários
/
Western Blotting
/
Reação em Cadeia da Polimerase
/
Baculoviridae
/
Vacinas Protozoárias
/
Clonagem Molecular
Idioma:
Inglês
Revista:
Southeast Asian J Trop Med Public Health
Ano de publicação:
1999
Tipo de documento:
Artigo
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