Partial purification and characterization of DNA topoisomerase II from Plasmodium falciparum.
Southeast Asian J Trop Med Public Health
;
1994 Mar; 25(1): 32-6
Artigo
em Inglês
| IMSEAR
| ID: sea-31380
ABSTRACT
DNA topoisomerase II from Plasmodium falciparum was partially purified by FPLC using three columns Econo-Pac Q, heparin-agarose and Mono Q. The enzyme showed ATP- and Mg2 +/- dependent activities in a decatenation assay, with optimum concentrations of 0.5 and 10 mM, respectively. Furthermore, highest activity was detected in the presence of 100 mM KCI. Enzyme decatenation activity was not inhibited by the DNA topoisomerase I inhibitor, camptothecin, but was sensitive to both prokaryotic and eukaryotic DNA topoisomerase II inhibitors.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Plasmodium falciparum
/
Células Procarióticas
/
Camptotecina
/
Eletroforese em Gel Bidimensional
/
Testes de Sensibilidade Microbiana
/
DNA de Protozoário
/
Cromatografia em Agarose
/
DNA Topoisomerases Tipo II
/
Fluoroquinolonas
/
Avaliação Pré-Clínica de Medicamentos
Idioma:
Inglês
Revista:
Southeast Asian J Trop Med Public Health
Ano de publicação:
1994
Tipo de documento:
Artigo
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