Long term maintenance of Toxoplasma gondii (Rh strain) in Vero cell line and use of harvested antigens for immunodiagnosis.
Southeast Asian J Trop Med Public Health
;
1991 Dec; 22 Suppl(): 124-8
Artigo
em Inglês
| IMSEAR
| ID: sea-31492
ABSTRACT
Thirty in vitro serial passages of Toxoplasman gondii cultures in Vero cell line performed once in every five days had a mean increase in parasite count of 74.4 +/- 14.8 times from that of initial counts. Long term cultures in Vero cell line did not alter the virulence of the parasite. The good correlation (r = 0.99) between the IFA titer and ELISA OD values using the parasite antigens from in vitro sources indicates that long term maintenance of T. gondii in culture does not affect significantly the ability to recognize antibodies to surface and soluble antigens. The results also show that soluble antigens containing host cells can be directly used for immunodiagnostic purposes without purification. The in vitro maintenance of T. gondii is safer and cheaper when compared to the in vivo method.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Toxoplasma
/
Virulência
/
Células Vero
/
Ensaio de Imunoadsorção Enzimática
/
Anticorpos Antiprotozoários
/
Inoculações Seriadas
/
Toxoplasmose Animal
/
Imunofluorescência
/
Animais
/
Camundongos
Idioma:
Inglês
Revista:
Southeast Asian J Trop Med Public Health
Ano de publicação:
1991
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS