Gene cloning and amino acid sequence analysis of triose-phosphate isomerase of Schistosoma japonicum Chinese strain.

ABSTRACT

A pair of primers was synthesized according to the DNA sequence of Schistosoma japonicum Philippine strain, and the mRNA of adult worms of S. japonicum Chinese strain was prepared. The gene of triose-phosphate isomerase of S. japonicum Chinese strain (SjC TPI) was successfully cloned from the mRNA through reverse transcription polymerase chain reaction (RT-PCR) technic with the primers. The DNA sequence of the gene showed that the open reading frame of encoding SjC TPI DNA includes 759bp, which has 84% homology to Sm TPI, 99.7% homology to SjP TPI. The analysis of deduced amino acid sequence of SjC TPI indicates that SjC TPI is 84.9% (214/252) identical with Sm TPI and 99.2% (250/252) with SjP TPI. The peptide-structure analysis presents 7 extra-surface, hydrophilic regions in the molecule of SjC TPI, the molecular weight of SjC TPI is 27,619. The isoelectric point is 7.38.