Effects of tunicamycin on the pH-activity pattern of acid phosphatase in Pseudomonas pseudomallei.
Southeast Asian J Trop Med Public Health
;
1994 Mar; 25(1): 144-51
Artigo
em Inglês
| IMSEAR
| ID: sea-31642
ABSTRACT
The liquid culture of Pseudomonas pseudomallei shows a complex feature in in the pH-activity pattern of acid phosphatase, not a single peak curve. There was an evident tendency that the higher activity shifted to the higher pH range with the growth of culture. The culture in the presence of tunicamycin (20 micrograms/ml) showed a decreased activity selectively in the higher pH range, while the activity in the lower pH was more heat-labile. The bacterial cells grown on agar plates containing tunicamycin were more heat-labile than the untreated control cells. The glucosidase-treatment reduced the enzymatic activity (of the phosphatase-active fractions from the living cells) with the shift of the optimum pH to lower pH. These observations together with some collateral findings suggest that the pH-activity pattern of acid phosphatase in P. pseudomallei is associated with the development of precursor enzyme proteins to mature glycoproteins.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Fosfatase Ácida
/
Tunicamicina
/
Fracionamento Celular
/
Burkholderia pseudomallei
/
Meios de Cultura
/
Avaliação Pré-Clínica de Medicamentos
/
Glucosidases
/
Temperatura Alta
/
Concentração de Íons de Hidrogênio
Idioma:
Inglês
Revista:
Southeast Asian J Trop Med Public Health
Ano de publicação:
1994
Tipo de documento:
Artigo
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