Immunodiagnosis of human fascioliasis using an antigen of Fasciola gigantica adult worm with the molecular mass of 27 kDa by a dot-ELISA.
Southeast Asian J Trop Med Public Health
;
2003 Dec; 34(4): 713-7
Artigo
em Inglês
| IMSEAR
| ID: sea-31846
ABSTRACT
Immunodominant antigens of an approximate molecular mass of 27 kDa (FG 27) were obtained from an excretory-secretory product of adult Fasciola gigantica by a simple continuous-elution method. A dot-ELISA using the FG 27 antigen was developed for the detection of specific antibodies from patients infected with F. gigantica. Control sera were obtained from patients with other parasitic infections and healthy volunteers. The accuracy, sensitivity, specificity, and positive and negative predictive values were 98.2%, 100%, 97.4%, 76.9% and 100%, respectively. This dot-ELISA is a specific, sensitive and easy to perform method for the rapid diagnosis of fascioliasis, particularly when more complex laboratory tests are unavailable.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Humanos
/
Ensaio de Imunoadsorção Enzimática
/
Valor Preditivo dos Testes
/
Sensibilidade e Especificidade
/
Fasciola
/
Fasciolíase
/
Animais
/
Antígenos de Helmintos
Tipo de estudo:
Ensaio Clínico Controlado
/
Estudo diagnóstico
/
Estudo prognóstico
Idioma:
Inglês
Revista:
Southeast Asian J Trop Med Public Health
Ano de publicação:
2003
Tipo de documento:
Artigo
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