Recombinant expression of Toxocara canis excretory-secretory antigen TES-120 in Escherichia coli.
Southeast Asian J Trop Med Public Health
;
2003 Dec; 34(4): 723-6
Artigo
em Inglês
| IMSEAR
| ID: sea-31910
ABSTRACT
The gene encoding the excretory-secretory antigen TES-120 of dog ascarid worm Toxocara canis was cloned into the bacterium Escherichia coli. The specificity of the recombinant TES-120 antigen produced by the bacterium was investigated. A total of 45 human serum samples from patients infected with differenthelminthes and protozoa, including 8 cases of toxocariasis, were tested against the recombinant antigens in immunoblot assays. The results from the assays revealed that the recombinant TES-120 antigen reacted with sera from toxocariasis patients only. This highly specific recombinant TES-120 antigen can potentially be used for the development of an inexpensive serodiagnostic assay for human toxocariasis.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Proteínas Recombinantes
/
Humanos
/
Larva Migrans
/
Immunoblotting
/
Proteínas de Helminto
/
Sensibilidade e Especificidade
/
Clonagem Molecular
/
Toxocara canis
/
Cães
/
Escherichia coli
Tipo de estudo:
Estudo diagnóstico
/
Estudos de avaliação
Idioma:
Inglês
Revista:
Southeast Asian J Trop Med Public Health
Ano de publicação:
2003
Tipo de documento:
Artigo
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