Advantage of Sandiford's counterstain in detection of Gram negative bacteria in clinical specimens.

Confirmation of bacterial in clinical specimens using Gram's stain with the Sandiford's modification was found to be more useful, especially when Gram-negative bacteria with other forms coexisted, than ordinary Gram's stain. It was useful for staining histopathological specimens also. We believe that this method deserves wider recognition and should be used as a standard procedure in laboratories.