Application of alcohol- or acetone-fixed Schistosoma eggs as an alternative to lyophilized eggs for the circumoval precipitin test.
Southeast Asian J Trop Med Public Health
;
1991 Sep; 22(3): 417-21
Artigo
em Inglês
| IMSEAR
| ID: sea-32445
ABSTRACT
Antigenicity of Schistosoma mansoni and S. japonicum eggs preserved in ethanol or acetone were assessed in a circumoval precipitin (COP) assay. The egg antigens were found to retain sufficiently their COP reactivity for the diagnosis of both schistosomiasis mansoni and japonica, although their reactivity became lower than that of lyophilized eggs. These alternative preparations for COP tests have advantages, such as keeping eggs directly in fixatives soon after the egg-purification process. Furthermore, evaporation-process may cause eggshell cleavages which facilitate the reaction. The possible usefulness of those eggs in COP assays in local endemic areas is discussed.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Acetona
/
Preservação Biológica
/
Esquistossomose mansoni
/
Esquistossomose Japônica
/
Humanos
/
Testes de Precipitina
/
Etanol
/
Estudos de Avaliação como Assunto
/
Liofilização
/
Antígenos de Helmintos
Tipo de estudo:
Estudo diagnóstico
/
Estudos de avaliação
Idioma:
Inglês
Revista:
Southeast Asian J Trop Med Public Health
Ano de publicação:
1991
Tipo de documento:
Artigo
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