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The value of fluorescence microscopy of auramine stained sputum smears for the diagnosis of pulmonary tuberculosis.
Southeast Asian J Trop Med Public Health ; 1998 Dec; 29(4): 860-3
Artigo em Inglês | IMSEAR | ID: sea-32469
ABSTRACT
Laboratory diagnosis of pulmonary tuberculosis rests on the bacteriological examination of sputum smears stained by the Ziehl-Neelsen (ZN) method for acid fast bacilli (AFB). In the present study, we have compared light microscopy of ZN stained smears with that of fluorescence microscopy of sputum smears stained by auramine-phenol flurochrome dye for detection of AFB in sputum specimens. Sputum specimens from a total of 2,600 clinically suspected and diagnosed cases of pulmonary tuberculosis were examined by both the methods. Sputum specimens from a total of 1,104 patients were found to be positive for AFB. These included sputa from 975 (37.5%) patients positive for AFB by both ZN and auramine staining methods and sputa from an additional 129 (4.96%) patients positive for AFB by auramine staining only. Thus auramine staining of sputum smears in comparison to that of ZN staining is a better method of sputum microscopy for demonstration of AFB in sputum specimens. Fluorescence microscopy is relatively more sensitive and has the added advantage of allowing a large number of sputum specimens to be examined in a given time, in laboratories equipped with a fluorescent microscope.
Assuntos
Texto completo: DisponíveL Índice: IMSEAR (Sudeste Asiático) Assunto principal: Escarro / Coloração e Rotulagem / Fatores de Tempo / Tuberculose Pulmonar / Benzofenoneídio / Humanos / Técnicas Bacteriológicas / Sensibilidade e Especificidade / Corantes / Microscopia Tipo de estudo: Estudo diagnóstico Idioma: Inglês Revista: Southeast Asian J Trop Med Public Health Ano de publicação: 1998 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: IMSEAR (Sudeste Asiático) Assunto principal: Escarro / Coloração e Rotulagem / Fatores de Tempo / Tuberculose Pulmonar / Benzofenoneídio / Humanos / Técnicas Bacteriológicas / Sensibilidade e Especificidade / Corantes / Microscopia Tipo de estudo: Estudo diagnóstico Idioma: Inglês Revista: Southeast Asian J Trop Med Public Health Ano de publicação: 1998 Tipo de documento: Artigo