Quantitative studies on the development of inoculated Brugia malayi microfilariae in Anopheles sinensis and Culex quinquefasciatus.

ABSTRACT

Definite numbers of B. malayi mf were inoculated into An. sinensis and Cx. quinquefasciatus, and their subsequent development was observed. The relationships between the dosage and the L3 positive rate on the one hand and the average filarial maturity rate on the other were defined. At the same dosages of 4 and 10 mf/mosquito, the L3 positive rates and the average filarial maturity rates in An. sinensis were much higher than those in Cx. quinquefasciatus (p less than 0.01). It was demonstrated that the immune response to filariae of the mosquitos was the main factor resulting in these differences. The intensity of the immune response not only varied with the species, but also declined with the aging of mosquitos. The results of our experiment might be useful to studies on the dynamics of filariasis transmission and the interaction between filariae and their insect hosts.