Partially purified antigens of Paragonimus heterotremus for serodiagnosis of human paragonimiasis.
Southeast Asian J Trop Med Public Health
;
1994 Mar; 25(1): 176-80
Artigo
em Inglês
| IMSEAR
| ID: sea-32985
ABSTRACT
The preparative crude extract of Paragonimus heterotremus was fractionated by isoelectric focusing. Fractions at pH 5 which contained a specific antigen with a relative molecular weight of 31.5 kDa were pooled and used in an indirect enzyme-linked immunosorbent assay (ELISA) and immunoblot analysis for diagnosis of human paragonimiasis. The sensitivity and specificity of ELISA were found to be 100% and 99% respectively. The band of 31.5 kDa antigenic component was found to give consistent reaction with paragonimiasis sera. The sensitivity, specificity and predictive value (positive and negative) of immunoblot analysis for the 31.5 kDa band were all 100%.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Paragonimíase
/
Paragonimus
/
Humanos
/
Ensaio de Imunoadsorção Enzimática
/
Testes Sorológicos
/
Immunoblotting
/
Fracionamento Celular
/
Sensibilidade e Especificidade
/
Concentração de Íons de Hidrogênio
/
Focalização Isoelétrica
Tipo de estudo:
Ensaio Clínico Controlado
/
Estudo diagnóstico
/
Estudo prognóstico
Idioma:
Inglês
Revista:
Southeast Asian J Trop Med Public Health
Ano de publicação:
1994
Tipo de documento:
Artigo
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