Cloning and heterologous expression of Cryptococcus neoformans CnSRB1 cDNA in Saccharomyces cerevisiae.
Southeast Asian J Trop Med Public Health
;
2008 May; 39(3): 484-91
Artigo
em Inglês
| IMSEAR
| ID: sea-33009
ABSTRACT
In this study, we report the results of cloning, sequencing and functional analysis by complementation test of the putative Cryptococcus neoformans homolog CnSRB1. The nucleotide sequence revealed 63% identity, and the deduced amino acid sequence showed 66 and 64% identity to its respective homolog of Saccharomyces cerevisiae and Candida albicans, respectively. Functional complementation test indicated that the putative CnSRB1 gene could compensate the defect caused by a mutation in ScSRB1 in the S. cerevisiae srb1 mutant. Taken together, these results suggest that the putative CnSrblp is a functional homolog of ScSrb1p.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Plasmídeos
/
Saccharomyces cerevisiae
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Candida albicans
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Proteínas Fúngicas
/
Homologia de Sequência do Ácido Nucleico
/
Northern Blotting
/
Southern Blotting
/
Reação em Cadeia da Polimerase
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Clonagem Molecular
/
DNA Complementar
Idioma:
Inglês
Revista:
Southeast Asian J Trop Med Public Health
Ano de publicação:
2008
Tipo de documento:
Artigo
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