Validation of osmotic fragility test and dichlorophenol indophenol precipitation test for screening of thalassemia and Hb E.
Southeast Asian J Trop Med Public Health
;
2005 Nov; 36(6): 1538-42
Artigo
em Inglês
| IMSEAR
| ID: sea-33151
ABSTRACT
The strategy for screening of thalassemia and Hb E by a combination of osmotic fragility (OF) test and dichlorophenol indophenol precipitation (DCIP) test was validated with 436 unrelated Thai subjects. Hemoglobin (Hb) typing, Hb A2 quantitation, PCR and DNA sequence analysis were used as confirmatory methods for diagnosis of thalassemia and hemoglobinopathy. The sensitivity and specificity of this strategy was 100% and 79.7%, respectively. The results assessed by two medical scientists were exactly the same with 93.3% accuracy in comparison with the confirmatory methods. A combination of OF and DCIP has been shown to be a reliable, rapid, simple and sensitive strategy for screening thalassemia and Hb E in the Thai population.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Talassemia
/
Humanos
/
Hemoglobina E
/
Testes Genéticos
/
Programas de Rastreamento
/
Reação em Cadeia da Polimerase
/
Valor Preditivo dos Testes
/
Análise de Sequência de DNA
/
2,6-Dicloroindofenol
/
Testes Hematológicos
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
/
Estudo de rastreamento
Idioma:
Inglês
Revista:
Southeast Asian J Trop Med Public Health
Ano de publicação:
2005
Tipo de documento:
Artigo
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