Sequence analysis of Orientia tsutsugamushi DNA from mites collected in the Xisa archipelago, China.
Southeast Asian J Trop Med Public Health
;
2002 Sep; 33(3): 551-6
Artigo
em Inglês
| IMSEAR
| ID: sea-33175
ABSTRACT
The genotype of Orientia tsutsugamushi DNA from mites in the Xisa archipelago of China were identified. A natural focus of tsutsugamushi disease in the archipelago was found. The DNA sequence that codes for the 56 kDa protein of O. tsutsugamushi was amplified by nested polymerase chain reaction (N-PCR). The purified positive products were cloned into a pGEM-T vector and sequenced. The DNA sequence was compared with various sequences on the internet for sequence homology. A 507 bp DNA fragment encoding the 56 kDa protein was amplified from the samples. The sequence homology was 85% (Karp strain), 68% (Gilliam strain), 65% (Kato strain), and 67% (Yonchon strain). Orientia tsutsugamushi is carried by the mites of the Xisa archipelago; the main genotype is the Karp strain.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Valores de Referência
/
Orientia tsutsugamushi
/
Polimorfismo de Fragmento de Restrição
/
Humanos
/
DNA Bacteriano
/
China
/
Técnicas de Tipagem Bacteriana
/
Genótipo
/
Animais
/
Ácaros
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Southeast Asian J Trop Med Public Health
Ano de publicação:
2002
Tipo de documento:
Artigo
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