In vivo culture of Cryptosporidium oocysts for laboratory use.
Southeast Asian J Trop Med Public Health
;
2006 ; 37 Suppl 3(): 18-20
Artigo
em Inglês
| IMSEAR
| ID: sea-33221
ABSTRACT
In the present study, we describe in vivo cultivation to produce oocysts. Seven-day-old mice were orally infected with 100,000-120,000 Cryptosporidium oocysts. On day 8 post-infection, the mice were killed by ether, and the small and large intestines collected. A simple extraction procedure was used and purified using Ficoll gradient centrifugation. After purification, the oocysts were preserved in phosphate buffered saline with antibiotic at 4 degrees C before use.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Contagem de Ovos de Parasitas
/
Doenças dos Roedores
/
Centrifugação com Gradiente de Concentração
/
Cryptosporidium
/
Meios de Cultura
/
Oocistos
/
Técnicas de Cultura
/
Fezes
/
Animais
/
Camundongos
Idioma:
Inglês
Revista:
Southeast Asian J Trop Med Public Health
Ano de publicação:
2006
Tipo de documento:
Artigo
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