Immunodiagnosis of clonorchiasis by enzyme-linked immunosorbent assay.
Southeast Asian J Trop Med Public Health
;
1988 Mar; 19(1): 117-21
Artigo
em Inglês
| IMSEAR
| ID: sea-33475
ABSTRACT
The present study applied the indirect enzyme-linked immunosorbent assay (ELISA) technique in the immunodiagnosis of clonorchiasis. Antigen used in this study was extracted from adult worms of Clonorchis sinensis obtained from cats. 132 patients with clonorchiasis, 100 healthy persons and 14 patients with other parasitic infections were studied. Mean O.D. ratio with standard deviation of clonorchiasis was 1.41 +/- 0.21 with 0.95 +/- 0.13 of healthy persons. Results revealed 90.2% to 95.5% of sensitivity and 84% to 99% specificity dependent on the two cut off values of O.D. ratio, i.e. 1.10 and 1.20. Antibody titers derived from O.D. ratio highly correlated with direct titration (Y = 0.0303 +/- 1.1766 X, r = 0.8945). Cross reactions of other parasite infections to clonorchiasis were observed in patients with angiostrongyliasis and schistosomiasis.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Doenças Parasitárias
/
Humanos
/
Ensaio de Imunoadsorção Enzimática
/
Testes Sorológicos
/
Anticorpos Anti-Helmínticos
/
Valor Preditivo dos Testes
/
Clonorquíase
/
Clonorchis sinensis
/
Animais
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Idioma:
Inglês
Revista:
Southeast Asian J Trop Med Public Health
Ano de publicação:
1988
Tipo de documento:
Artigo
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