Development of cultivation technique for pure Plasmodium falciparum gametocytes.
Southeast Asian J Trop Med Public Health
;
1995 Dec; 26(4): 606-10
Artigo
em Inglês
| IMSEAR
| ID: sea-33507
ABSTRACT
Pure gametocyte culture of Plasmodium falciparum, isolate KT3, from Kanchanaburi Province, Thailand, was successfully established by 5% sorbitol treatment on days 9, 10 and 11 following initiation of culture. Using medium supplemented with 15% human plasma and activated erythrocytes, daily medium change was not required during the cultivation. There was 99% reduction in the numbers of asexual parasites in the culture but the numbers of gametocytes were not affected. Furthermore, the gametocytes could undergo their usual morphological development with retention of function as demonstrated by the appearance of exflagellating microgametocytes, macrogametocytes and of oocyst formation in midgut of infected mosquito.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Plasma
/
Plasmodium falciparum
/
Sorbitol
/
Fatores de Tempo
/
Feminino
/
Humanos
/
Masculino
/
Malária Falciparum
/
Meios de Cultura Livres de Soro
/
Eritrócitos
Idioma:
Inglês
Revista:
Southeast Asian J Trop Med Public Health
Ano de publicação:
1995
Tipo de documento:
Artigo
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