Comparative analysis of serological and molecular results for HLA-DR typing in 120 Thai subjects.
Southeast Asian J Trop Med Public Health
;
1999 Jun; 30(2): 311-3
Artigo
em Inglês
| IMSEAR
| ID: sea-33813
ABSTRACT
In this study, serological HLA-DR typing results were compared to typing results obtained with sequence-specific primers in the polymerase chain reaction (PCR-SSP). HLA-DR typing was performed on 120 random Thai individuals. Differences in HLA-DR typing results were found in 18 out of 120, which were due to cross reactive antibodies and the lack of potent antisera to define proper HLA-DR splits by serology. Furthermore, PCR-SSP is fast and easy to perform as HLA-DR typing results can be obtained within 2 hours. From the results of this study it can be concluded that PCR-SSP is a reliable and promising technique for HLA-DR typing which can replace the serological technique in routine clinical practice.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Tailândia
/
Humanos
/
Teste de Histocompatibilidade
/
Testes Sorológicos
/
Antígenos HLA-DR
/
Reação em Cadeia da Polimerase
/
Reprodutibilidade dos Testes
/
Sensibilidade e Especificidade
/
Primers do DNA
/
Anticorpos Monoclonais
Tipo de estudo:
Estudo diagnóstico
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Southeast Asian J Trop Med Public Health
Ano de publicação:
1999
Tipo de documento:
Artigo
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