Protein A-antibody mediated hemagglutination assay for serodiagnosis of amebic liver abscess.
Southeast Asian J Trop Med Public Health
;
1991 Jun; 22(2): 249-53
Artigo
em Inglês
| IMSEAR
| ID: sea-33852
ABSTRACT
A successful modification of the indirect hemagglutination test to demonstrate antibodies for serodiagnosis of amebic liver abscess has been described in the present study. In the modified test, the protein A-IMA, Staphylococcus aureus (Cowan's strain I) bearing protein A (SAPA) cells were used to enhance hemagglutination of sensitized red cells. Use of SAPA cells markedly enhanced sensitivity of the test and greatly increased the titers obtained with most of the sera. At a diagnostic antibody titer of 1128 and above, the protein A-IHA could detect 72 (100%) of amebic liver abscess (ALA) cases. Amongst the controls, no false positive reaction was observed in non-amebic liver disease controls. However 1(2%) of sera demonstrated false positive reactions from healthy controls. The protein A-IHA was highly sensitive when compared with that of the indirect hemagglutination (IHA) for serodiagnosis of amebic liver abscess. The novel immunoassay is simple, inexpensive and requires little technical skill. It has the potential for wide application in serodiagnosis of amebic liver abscess.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Proteína Estafilocócica A
/
Staphylococcus aureus
/
Humanos
/
Testes de Hemaglutinação
/
Anticorpos Antiprotozoários
/
Sensibilidade e Especificidade
/
Entamoeba histolytica
/
Abscesso Hepático Amebiano
/
Animais
/
Antígenos de Protozoários
Tipo de estudo:
Estudo diagnóstico
Idioma:
Inglês
Revista:
Southeast Asian J Trop Med Public Health
Ano de publicação:
1991
Tipo de documento:
Artigo
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