Effects of lead on the proliferation, protein production, and osteocalcin secretion of human dental pulp cells in vitro.
Southeast Asian J Trop Med Public Health
;
2002 Sep; 33(3): 654-61
Artigo
em Inglês
| IMSEAR
| ID: sea-34127
ABSTRACT
Teeth have been recognized as providing a useful long-term record of lead (Pb2+) uptake. However, information regarding the effects of lead on dental pulp tissue cells that foster dentinogenesis is scarce. This study investigated the effects of lead on dental pulp tissue using human dental pulp fibroblasts in vitro. Dental pulp cells from the teeth of young patients (aged 17-24 years) were cultured and subsequently treated with lead glutamate. It was shown that, in serum-free conditions, all the tested concentrations of lead (4.5 x 10(-5) M, 4.5 x 10(-6) M, and 4.5 x 10(-7) M) significantly increased pulpal cell proliferation. In the presence of 2% fetal bovine serum, increasing cell proliferation was observed only after exposure to a lead concentration of 4.5 x 10(-5) M. However, protein, procollagen type I, and osteocalcin productions were significantly decreased. The alteration of cell population and protein production of affected human dental pulp shown in this study are toxic effects of the lead.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Biossíntese de Proteínas
/
Humanos
/
Proteínas
/
Osteocalcina
/
Divisão Celular
/
Células Cultivadas
/
Análise de Variância
/
Adolescente
/
Adulto
/
Colágeno Tipo I
Idioma:
Inglês
Revista:
Southeast Asian J Trop Med Public Health
Ano de publicação:
2002
Tipo de documento:
Artigo
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