Validation of ELISA test kits for detection of beta-agonist residues in meat.
Southeast Asian J Trop Med Public Health
;
2004 Jun; 35(2): 481-7
Artigo
em Inglês
| IMSEAR
| ID: sea-34308
ABSTRACT
Three ELISA test kits, the Randox ELISA beta-agonist test kit, Euro-Diagnostica test kit, and Ridascreen beta-agonist test kit, were evaluated for screening of meat and liver for beta-agonist residues in fortified and field-incurred samples. It was found that the Randox beta-agonist test kit was more suitable as a screening tool due to its accuracy, ease of use, and lower cost. The tests were able to detect beta-agonist residues at the minimum level of detection, as claimed by the suppliers. The performance of the method as assessed through recovery rates of beta-agonists in fortified samples was satisfactory with a low coefficient of variation (1-3%). Repeatability, as measured through the coefficient of correlation was also satisfactory. For field-incurred positive samples, the test kit showed a sensitivity of 100% and a low rate of false positives for goat and cow tissues. However, a high rate of apparent false positives was obtained for tissues of swine.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Suínos
/
Bovinos
/
Ensaio de Imunoadsorção Enzimática
/
Cabras
/
Resíduos de Drogas
/
Contaminação de Alimentos
/
Agonistas Adrenérgicos beta
/
Drogas Veterinárias
/
Fígado
/
Animais
Tipo de estudo:
Estudo diagnóstico
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Southeast Asian J Trop Med Public Health
Ano de publicação:
2004
Tipo de documento:
Artigo
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