Pineapple juice for digestion of swamp eel viscera for harvesting infective-stage larva of Gnathostoma spp.
Southeast Asian J Trop Med Public Health
;
2004 Jun; 35(2): 267-9
Artigo
em Inglês
| IMSEAR
| ID: sea-34325
ABSTRACT
Third-stage larvae were used as antigen in the diagnosis of gnathostomiasis in Western blot analysis. Normally, the larvae were obtained from digestion of eel's liver (Fluta alba) by the enzyme pepsin. We used pineapple juice (Ananus comosus) instead of enzyme pepsin in harvesting Gnathostoma spinigerum third-stage larvae. The difference in recovered larvae numbers, between pineapple juice and pepsin, were not statistically significantly different (p>0.05). The larvae from pepsin and pineapple juice digestion were cultivated on BME for 7 days; the survival rates were not significantly different (p>0.05). Thus, pineapple juice is another enzyme of choice for recovering Gnathostoma spinigerum third-stage larvae.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Soluções
/
Bebidas
/
Larva Migrans Visceral
/
Western Blotting
/
Parasitologia de Alimentos
/
Pepsina A
/
Infecções por Spirurida
/
Ananas
/
Digestão
/
Enguias
Idioma:
Inglês
Revista:
Southeast Asian J Trop Med Public Health
Ano de publicação:
2004
Tipo de documento:
Artigo
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