Isolation and PCR detection of rickettsiae from clinical and rodent samples in Malaysia.
Southeast Asian J Trop Med Public Health
;
2002 Dec; 33(4): 772-9
Artigo
em Inglês
| IMSEAR
| ID: sea-34415
ABSTRACT
Isolation of rickettsiae from patients' blood samples and organ samples of wild rodents from areas with high seroprevalence of rickettsial infections was attempted using cell culture assay and animal passages. L929 mouse fibroblast cells grown in 24 well tissue culture plate were inoculated with buffy coat of febrile patients and examined for the growth of rickettsiae by Giemsa, Gimenez staining and direct immunofluorescence assay. No rickettsiae were isolated from 48 patients' blood samples. No symptomatic infections were noted in mice or guinea pigs infected with 50 organ samples of wild rodents. There was no rickettsial DNA amplified from these samples using various PCR detection systems for Orientia tsutsugamushi, typhus and spotted fever group rickettsiae.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Rickettsia
/
Infecções por Rickettsia
/
Doenças dos Roedores
/
Roedores
/
Corantes Azur
/
Idoso
/
Feminino
/
Humanos
/
Masculino
/
DNA Bacteriano
Tipo de estudo:
Estudo diagnóstico
/
Estudo de rastreamento
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Southeast Asian J Trop Med Public Health
Ano de publicação:
2002
Tipo de documento:
Artigo
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