Analysis of the reg1alpha and reg1beta gene transcripts in patients with fibrocalculous pancreatopathy.
Southeast Asian J Trop Med Public Health
;
2002 Jun; 33(2): 365-72
Artigo
em Inglês
| IMSEAR
| ID: sea-34565
ABSTRACT
Fibrocalculous pancreatopathy is a form of diabetes, associated with tropical chronic calcific pancreatitis, in which islet beta-cell loss and pancreatic stone formation are found. It is likely to be a multifactorial disease with both genetic and environmental components. Regenerating (reg) gene encodes protein that has been involved in pancreatic lithogenesis and the regeneration of islet cells and therefore the abnormality of reg genes could be associated with fibrocalculous pancreatopathy. In this study, regla and reg1beta mRNAs were isolated from peripheral blood lymphocytes obtained from 16 patients with fibrocalculous pancreatopathy, 42 patients with type 1 diabetes, 37 patients with type 2 diabetes, and 22 normal controls. mRNAs were amplified by reverse-transcription polymerase chain reaction (RT-PCR) and analysed by a single strand conformation polymorphism (SSCP) technique. The reg1alpha and reg1beta mRNAs were isolated, indicating the ectopic expression of these genes in peripheral blood lymphocytes; however, variation among mobility patterns was not observed in the SSCP analysis of the RT-PCR products. The results indicated that there was no abnormality of the regla and reg1beta mRNAs obtained from the study groups.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Pancreatopatias
/
Tailândia
/
Humanos
/
Proteínas de Ligação ao Cálcio
/
RNA Mensageiro
/
Enzimas de Restrição do DNA
/
Reação em Cadeia da Polimerase
/
Polimorfismo Conformacional de Fita Simples
/
Eletroforese em Gel de Ágar
/
Litostatina
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Southeast Asian J Trop Med Public Health
Ano de publicação:
2002
Tipo de documento:
Artigo
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