Sensitive enzyme-linked immunosorbent assay with urine samples: a tool for surveillance of schistosomiasis japonica.
Southeast Asian J Trop Med Public Health
;
2003 Sep; 34(3): 469-72
Artigo
em Inglês
| IMSEAR
| ID: sea-34710
ABSTRACT
An enzyme-linked immunosorbent assay (ELISA) to detect antibodies to Schistosoma japonicum soluble egg antigens (SEA) in un-concentrated urine was developed. The urine ELISA was applied to samples collected in a schistosomiasis-endemic village in China. The levels of anti-SEA antibodies detected in urine correlated well with those obtained with paired serum samples (r = 0.694, p<0.0001). Among 129 serum ELISA positives, 112 (86.8%) were positive by urine ELISA, while all 40 serum ELISA negatives from a non-endemic area were negative. The levels of anti-SEA in urine samples were stable up to 8 weeks of storage at 37 degrees C, with sodium azide as a preservative. Therefore, ELISA with urine samples can be used for the surveillance of schistosomiasis.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Manejo de Espécimes
/
Esquistossomose Japônica
/
Humanos
/
Imunoglobulina G
/
Ensaio de Imunoadsorção Enzimática
/
Anticorpos Anti-Helmínticos
/
Vigilância da População
/
Sensibilidade e Especificidade
Tipo de estudo:
Estudo diagnóstico
/
Estudos de avaliação
/
Estudo de rastreamento
Idioma:
Inglês
Revista:
Southeast Asian J Trop Med Public Health
Ano de publicação:
2003
Tipo de documento:
Artigo
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