Genetic differences of E gene of a Thai strain of Japanese encephalitis virus that determine small plaque size phenotype but not neurovirulence in suckling mice.
Southeast Asian J Trop Med Public Health
;
2008 May; 39(3): 387-93
Artigo
em Inglês
| IMSEAR
| ID: sea-34940
ABSTRACT
Two small plaque variants of Japanese encephalitis virus (JEV), S4P9 and S9P10, were recovered from the wild type of JEV strain KE-093 using plaque purification in combination with the temperature-shift induction technique. Growth patterns of the S4P9 and S9P10 in BHK-21 cells as well as neurovirulence in suckling mice were similar to that of KE-093. An amino acid substitution, lysine for glutamic acid, was present in envelope protein at residue E-83 in the small plaque variants. This study shows that small plaque phenotype is not always associated with attenuation in vivo.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Fenótipo
/
Ensaio de Placa Viral
/
Tailândia
/
Variação Genética
/
Virulência
/
Humanos
/
Glicoproteínas de Membrana
/
Ácidos Nucleicos
/
Proteínas do Envelope Viral
/
Vírus da Encefalite Japonesa (Espécie)
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Southeast Asian J Trop Med Public Health
Ano de publicação:
2008
Tipo de documento:
Artigo
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