RNA in situ hybridization for the detection of Feline Immunodeficiency Virus in infected cells.
Southeast Asian J Trop Med Public Health
;
2006 Jan; 37(1): 106-12
Artigo
em Inglês
| IMSEAR
| ID: sea-35014
ABSTRACT
In the present study, we have applied a novel approach to generate specific digoxigenin- and biotin-labeled RNA probes to detect Feline Immunodeficiency Virus (FIV) gag gene in the FIV-infected feline T-lymphoblastoid cell line (MYA-1). This involved cloning of the FIV gag gene into a PCR Script vector containing both T3 and T7 promoters, followed by amplification of the insert and the two promoter sequences, using specific primer sequences. The FIV RNA probes were more sensitive than FIV DNA probes. This approach should make RNA in situ hybridization more accessible for use in routine diagnosis.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Coloração e Rotulagem
/
Virologia
/
Biotina
/
Linfócitos
/
Sondas de DNA
/
Sondas RNA
/
Gatos
/
Genes gag
/
Reação em Cadeia da Polimerase
/
Regiões Promotoras Genéticas
Tipo de estudo:
Estudo diagnóstico
Idioma:
Inglês
Revista:
Southeast Asian J Trop Med Public Health
Ano de publicação:
2006
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS