Methylene blue staining method for identification of Opisthorchis viverrini egg.
Southeast Asian J Trop Med Public Health
;
2005 ; 36 Suppl 4(): 107-9
Artigo
em Inglês
| IMSEAR
| ID: sea-35287
ABSTRACT
Methylene blue staining method was used to distinguish O. viverrini eggs from Haplorchis taichui and Prosthodendrium molenkampi eggs. All eggs were obtained from dissected adult worms, fixed in 10% formalin, and stained with methylene blue prior to light microscopy observation. The distinct musk-melon-like texture of the O. viverini eggshell surface and the thread-like texture of H. taichui eggshell surface were recognized, while P. molenkampi eggs showed a smooth eggshell. We also evaluated the sensitivity and specificity of the method by training investigators to differentiate surface textures. After training, the investigators were randomly tested with 10 slides containing fluke eggs. The sensitivity and specificity were 95% and 95%, respectively.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Opistorquíase
/
Opisthorchis
/
Óvulo
/
Especificidade da Espécie
/
Coloração e Rotulagem
/
Humanos
/
Sensibilidade e Especificidade
/
Corantes
/
Animais
/
Azul de Metileno
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Idioma:
Inglês
Revista:
Southeast Asian J Trop Med Public Health
Ano de publicação:
2005
Tipo de documento:
Artigo
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