A recombinant immunodiagnostic antigen for bovine cysticercosis.
Southeast Asian J Trop Med Public Health
;
1991 Dec; 22 Suppl(): 268-70
Artigo
em Inglês
| IMSEAR
| ID: sea-35407
ABSTRACT
The 70% ammonium sulfate-soluble fraction of the cyst fluid of Taenia hydatigena (designated ThFAS) was previously shown to have potential as an immunodiagnostic reagent for bovine cysticercosis. Western blot analysis indicated that the specific reactivity with antibodies in sera of T. saginata-infected cattle was associated with a 10 kDa component. Rabbit antiserum to ThFAS identified a homologous antigenic protein from the cestode Taenia crassiceps. Consequently, a cDNA expression library was constructed in lambda gt11 using poly A mRNA purified from T. crassiceps metacestodes and screened with rabbit antiserum to ThFAS. One strongly reactive clone (designated lambda TCA-2) produced a 123 kDa beta-galactosidase fusion protein which reacted in Western blot with sera from calves experimentally-infected with T. saginata and did not react with sera from uninfected calves or from cattle infected with Fasciola hepatica or with common gastrointestinal cattle parasites.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Taenia
/
Proteínas Recombinantes
/
DNA
/
Bovinos
/
Cisticercose
/
Anticorpos Anti-Helmínticos
/
Doenças dos Bovinos
/
Biblioteca Gênica
/
Western Blotting
/
Animais
Tipo de estudo:
Estudo prognóstico
Idioma:
Inglês
Revista:
Southeast Asian J Trop Med Public Health
Ano de publicação:
1991
Tipo de documento:
Artigo
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