Determination of subgroup, serotype and electropherotype of human rotaviruses obtained from diarrheic patients in the Philippines.
Southeast Asian J Trop Med Public Health
;
1990 Jun; 21(2): 249-58
Artigo
em Inglês
| IMSEAR
| ID: sea-35837
ABSTRACT
A total of 40 rotavirus specimens obtained from infants with acute gastroenteritis in Metro Manila, Philippines, were analyzed for their subgroup and serotype specificities and RNA electropherotype. A half (50%) of the specimens contained subgroup I human rotaviruses (HRVs), while 45% of them contained subgroup II HRVs. All subgroup II HRVs were determined to be serotype 1, except one specimen belonging to serotype 3, by an enzyme-linked immunosorbent assay with serotype specific monoclonal antibodies. However, serotype specificity of all subgroup I specimens failed to be determined. In polyacrylamide gel electrophoresis, RNAs extracted from HRVs of either subgroup showed long electropherotype. At least two electropherotypes were distinguished in subgroup I viruses, while five different patterns of viral RNA were found in subgroup II viruses.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Filipinas
/
Infecções por Rotavirus
/
Feminino
/
Humanos
/
Masculino
/
Recém-Nascido
/
RNA
/
Ensaio de Imunoadsorção Enzimática
/
Eletroforese em Gel Bidimensional
/
Sorotipagem
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Southeast Asian J Trop Med Public Health
Ano de publicação:
1990
Tipo de documento:
Artigo
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