Sensitive detection of Pneumocystis jirovecii DNA in gastric wash using nested polymerase chain reaction.
Southeast Asian J Trop Med Public Health
;
2007 Sep; 38(5): 892-6
Artigo
em Inglês
| IMSEAR
| ID: sea-35838
ABSTRACT
One hundred and five samples of gastric washes were obtained from 52 pediatric patients. Eleven of the 105 samples (10%) gave positive results using immunofluorescence antibody test (IFA) for Pneumocystis jirovecii. Single-step polymerase chain reaction (PCR) produced 13% (14 samples), whereas detection by nested PCR was increased to 65 samples (62%). Moderate agreement (kappa = 0.5) was found between test results of IFA and single-step PCR, but no agreement was found between the results of IFA and nested PCR (kappa = 0.1).
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Pneumonia por Pneumocystis
/
Humanos
/
Criança
/
Reação em Cadeia da Polimerase
/
Imunofluorescência
/
Sensibilidade e Especificidade
/
Pneumocystis carinii
/
Mucosa Gástrica
Tipo de estudo:
Estudo diagnóstico
Idioma:
Inglês
Revista:
Southeast Asian J Trop Med Public Health
Ano de publicação:
2007
Tipo de documento:
Artigo
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